SureSilencing™ siRNA Arrays

The SureSilencing siRNA Array is the latest technological innovation for conducting functional studies on your pathway of interest through RNA interference (RNAi). Validated siRNA (2 sequences per gene) for 42 key pathway-focused genes with appropriate RNAi controls are arrayed on a 96-well plate. Following a simple reverse transfection protocol with the siRNA, you can directly determine the phenotype of the cells on the same plate with colorimetric, luminescent or fluorescent cell-based assays. Using the SureSilencing siRNA Arrays, you can quickly identify genes involved in a biological process or disease state, the targets of chemical compounds, and the biological impact of your genes of interest in a pathway.

Pathway List:

Why siRNA Arrays?

• Pathway Focused: Knock down 42 key genes in the same biological pathway
• Reliable and Proven: All siRNA sequences are experimentally validated to deliver > 70% knockdown* and designed to minimize off-target effects.
• Ease-of-Use & High-Performance: Simple reverse transfection protocol, convenient plate design for cell-based assays, and high-efficiency transfection reagent make the functional study of pathways accessible to everyone.
• Pathway-Powered Data Analysis: Comparative Knockdown Analysis from Your Array.

Performance and Applications

Performance

Specificity: Minimizing Off-Target Effects
Our stringent computer algorithm minimizes off-target effects by using a sophisticated sequence alignment filter to design gene-specific siRNA. It also includes filters for all of the published physical and sequence properties known to be important for siRNA activity.

Efficacy: Validated & Guaranteed Knock Down for Every Gene on Each Array
Each siRNA pair is validated experimentally on the bench for greater than 70 percent knock down minimizing false negatives. You can be sure that the lack of a phenotype for a given gene is NOT due to a lack of gene knockdown.

Applications
SureSilencing siRNA Arrays are designed to study gene function with cell-based assays such as fluorescence assays for cell viability, chemiluminescence assays for enzyme activity, luciferase reporter assays for pathway activation, colorimetric assays for protein phosphorylation, and microscopy for morphology & immunostaining. The SureSilencing siRNA Arrays may be used for accurate pathway analysis, the functional analysis of novel genes, and drug target, enhancer, or inhibitor validation.
View Two SureSilencing siRNA Array Application Examples

How It Works

Simple Workflow

1. Add medium and transfection agent to wells pre-coated with siRNA.
2. Plate trypsinized cells in suspension into the wells.
3. Analyze phenotype using cell-based assays.

The reverse transfection method produces equivalent or improved transfection efficiency over standard pre-plated methods** and saves an entire day in the process. The simplicity and reliability of the siRNA Array allows you to quickly analyze gene function and mechanism of action in your pathways of interest.
See the SureSilencing siRNA Array Protocol Overview

Well Designed Layout and Controls
Each siRNA Array contains duplicate wells of siRNA pairs for 42 pathway-focused genes, and four replicate RNA interference and phenotypic assay controls including negative control siRNA, mock transfection, transfection efficiency, and assay background.
See the Layout of the siRNA Array & Learn More About the Control Wells

PRODUCT SUPPORT

Technical Support

Presentations

* The SureSilencing siRNA Arrays are guaranteed to knock down expression of the targeted genes at the RNA level by at least 70 percent as measured by real-time qRT-PCR under conditions of consistently high transfection efficiencies.

** Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature 411:107-110.

This product is made under license from The Carnegie Institution of Washington. However, the purchase of this material by a non-academic or for-profit organization will require a license to use the material from Carnegie. License queries may be directed to Gloria Brienza or Gary Kowalczyk at The Carnegie Institution of Washington, 1530 P Street NW, Washington, DC 20005.