Why RT² qPCR Primer Assays?

• High performance: Each RT² qPCR Primer Assay is experimentally validated to amplify a single amplicon of the correct size with uniform PCR efficiency. The performance of RT² qPCR Primer Assays for gene expression analysis is as good as or better than that of TaqMan® Assays.
• Complete genome coverage: Primer assays are available for every human, mouse, rat, rhesus macaque, or even fruit fly gene. The uniform PCR efficiency and PCR conditions of RT² qPCR Primer Assays allow for an easy transition from single gene analysis to multiple gene expression analyses.
• Convenience: With less than 5-minutes of hands-on time, RT² qPCR Primer Assays deliver guaranteed performance when used with SABiosciences' optimized instrument-specific RT² qPCR master mixes for ABI, Bio-Rad, Stratagene and other real-time PCR instruments.

Learn more about RT² qPCR Primer Assays, download our technology brochure.

How It Works
The RT² qPCR Primer Assay is the most reliable SYBR® Green-based quantitative real-time PCR assay for gene expression analysis. Our experimentally verified design algorithm yields gene-specific qPCR assays characterized by uniform and high PCR efficiencies and standardized amplification conditions. Every RT² qPCR Primer Assay is subjected to rigorous experimental verification. Single product amplification of the correct size and high PCR efficiency are guaranteed when using the appropriate RT² qPCR master mixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.

Necessary qPCR Reagents for Guaranteed Performance:

• RT² qPCR master mix specially formulated and optimized for your real-time PCR instrument. Click [here] to select your RT² qPCR Primer Assays.

Other Reagents to Be Considered

• Housekeeping genes for normalization
• RT² First Strand Kit

Learn more about RT² Real-Time™ PCR technologies [here].

Read a White Paper about validating microarray results with real-time PCR [here].

For more technical information about out RT² qPCR Primer Assays, see the User Manual.