ChampionChIP qPCR Primers are available for studying the promoter region of every human, mouse, and rat gene. These primers are specifically designed and experimentally validated for SYBR Green based real-time PCR. They are optimized to measure genomic DNA enrichment among ChIP-purified DNA samples. These primers are ready to use for studying transcriptional regulation of your favorite genes.
ChampionChIP Real-Time PCR Primers are designed to detect specific genomic
DNA sequences within a 30 kb promoter region around every transcription start
site (TSS) in the RefSeq database. An average 1-kb tiling interval, when coupled
with an average ChIP DNA fragmentation size of 1 kb, provides a balance between
sensitivity and resolution. The genome-wide coverage of the ChampionChIP PCR
Primers provides a rapid screening assay for many target loci in the genome.
ChampionChIP PCR Primers meet all the essential requirements for accurate and
reliable delta-delta Ct calculations for absolute fractional enrichment of a
target nuclear factor at a specific promoter site as well as relative
fold-change differences across multiple samples. For details on these
calculations and on interpreting the results, see our ChIP-qPCR
Assay Data Analysis Template.
Start your search by choosing a species and entering one gene identifier into
the appropriate dialog box below:
Transcription Factor Researchers:
Use this search portal to find PCR primers for your favorite transcription
factors' binding sites if you know their location relative to the TSS of your
genes of interest.
This search portal returns all 30 available primers tiled around the TSS
of the queried gene.
Use the drop-down button to select the tile closest to your binding site
and see the relative position of the primers.
Because primer designs fall anywhere within a specified 1-kb tile region,
the closest assay to your binding site of interest may actually lie in an
adjacent tile. Contact technical support with any questions.
Histone Modification Researchers:
Use this search portal to identify PCR primers for specific regions in your
favorite gene bound by modified histones. For example:
The primers for the +1 and -1 kb tiles are often used to define two major
groups of histone modifications related to transcriptional activation and
repression of most genes.
The primers for all thirty tiles in a gene's promoter may be used in a Custom
Promoter Binding PCR Array to discover the binding positions of
different modified histones across that gene's promoter.
