RT² Real-Time PCR Assays

 

PCR Arrays

miRNA PCR Arrays

SYBR Single Gene Assays

RT² Real-Time PCR Assays   SABiosciences, a leader in the field of high-performance SYBR Green real-time PCR analysis, offers comprehensive gene expression services based on RT² qPCR Primer Assays that are available for every human, mouse and rat gene. Each RT² qPCR Primer Assay has been experimentally validated to amplify a single amplicon of the correct size with uniform PCR efficiency. In addition, the service core offers custom design and validation services enabling analysis of any gene regardless of species. SABiosciences' validated procedures and optimized processes yield the highest data quality and fastest turn-around time. Learn more about RT² Real-Time PCR technologies. For FFPE (formalin-fixed paraffin-embedded) samples, LCM (laser capture microdissection), FNAB (fine needle aspiration biopsies) and other small samples, require special isolation procedures and may require pre-amplification. Description of Services RNA isolation and QC (optional) Individual qRT-PCR assay service. 100% validated, available for all human, mouse and rat genes Custom design and validation service available for other species genes, or other types of projects (viral, bacterial etc.)

Sample Processing

Data Delivery

Pricing

Submitted Samples are Cells or Tissue:      Option of RNA isolation service      Recommended Sample Isolation and Storage Procedure Submitted Samples are Total RNA:      Recommended RNA Isolation methods      Quantification and estimating RNA purity using a NanoDrop spectrophotometer      Assessing RNA quality using an Agilent 2100 Bioanalyzer with a RNA 6000 NanoChip Reverse-transcription using our RT² First Strand Kit Real-time detection on cataloged or custom PCR array Data analysis using our PCR Array data analysis software Data report delivered via email or secure FTP site Back to Top

Sample Processing

Data Delivery

Pricing

Data analysis is performed using our qRT-PCR data analysis software. Data in an electronic form can be downloaded from a secure FTP website or delivered via email. A complete data and experiment report includes: Raw Ct values ΔΔCt Analysis Excel spreadsheet View a sample data set (.PDF) (email: support@sabiosciences.com) Custom data format service is also available. Back to Top

Sample Processing

Data Delivery

Pricing

Reverse Transcription prices start from $50 per sample and include the minus-RT control. ΔΔCt Service $ 50 per sample per gene (triplicate reactions performed) Gene quantity counts must include a customer-selected housekeeping control gene. Initial Set Up and Consultation: $300 per project. Contact a Technical Support representative to order or to discuss your specific need at support@sabiosciences.com or 301-682-9200. Prices include the cost of arrays, reagents and data analysis. Service prices are volume-dependent. Progressive discounts for fees and services are available. All prices are in US Dollars. Prices are subject to change at any time without notice. Back to Top