White Papers
Detailed Technical Resources about SABiosciences Technologies

PCR Arrays and Real-Time PCR

PCR Array: A Simple and Quantitative Method for Gene Expression Profiling
This paper evaluates the performance of the latest technique for monitoring the expression of a panel of pathway- or disease focused genes: the RT² Profiler PCR Array System. It presents scientific data showing that PCR Arrays have the sensitivity, reproducibility, and specificity expected from real-time PCR techniques.

PCR Array Application Examples: Toxicology, Oncology, and Immunology Research
This paper provides several application examples for the latest technique for monitoring the expression of a panel of pathway- or disease focused genes: the RT² Profiler PCR Array System. It explores the use of the PCR Arrays in immunology, cancer, and toxicology research.

Biomarker Discovery from Paraffin Embedded Samples
In this article, we present the successful principles and results of the new RT² FFPE PreAMP™ technology. This technology combines a novel RNA extraction protocol that exploits thermodynamic and structural characteristics of FFPE RNA with a powerful preamplification step of a pathway-focused set of genes for real-time gene expression analysis.

A Systematic Guideline for Developing Real-time PCR Primers
This paper summarizes the important principles that we have learned after designing real-time PCR primer assays for over 14,000 genes. Critical steps in designing and validating real-time PCR primers and the solutions to potential problems are described.

RT² miRNA PCR Arrays & Assays

The Complete System for Genome-Wide & Pathway Focused microRNA Analysis
This paper discusses some major obstacles in miRNA real-time PCR assay development and evaluates the performance of the newest technique for the analysis of genome-wide miRNA expression: RT² miRNA PCR Arrays from SABiosciences.

RNA Interference

Did Your RNAi Experiment Work?! Reliably Validating RNA Interference with Real-Time PCR
This white paper addresses the sources of variation the must be dealt with in validating RNAi. It also describes how the design algorithm for the SureSilencing shRNA Plasmids has twice the success rate of a more commonly used resource, and how it can guarantee the delivery of two successful designs per gene.

Microarrays

Oligo GEArrays: The Pathway Focused DNA Microarrays
This paper describes the flexible and easy-to-use technology and sensitive, reproducible, and reliable performance characteristics of the pathway-focused Oligo GEArray DNA microarray system, an effective alternative to genome-wide microarrays.

Validating Microarray Data Using RT² Real-Time™ PCR
This paper describes the use of real-time PCR for the confirmation of microarray data. A complete walk-through of a protocol using RT2 qPCR Primer Assays is provided along with discussion of the considerations necessary for a complete and successful experiment.

DNA Microarray Profiling From As Few As 20 Cells: The TrueLabeling-PicoAMP Kit
This paper describes an easier and more complete method for amplifying picogram quantities of total RNA (from LCM, FNAB, and FACS) for gene expression profiling studies, making microarray-based analyses possible for researchers working with these limited amounts of biological source material.

RNA Isolation

Improved Sensitivity for FFPE Microarray Analysis: ArrayGrade™ FFPE RNA Isolation
This paper describes a new method for isolating RNA from formalin-fixed paraffin-embedded (FFPE) blocks and slides that improves sensitivity and the number of positive calls in both high-density genome-wide and low-density pathway-focused microarray techniques, bringing gene expression profiling to researchers with access to archival tissue samples.

Reporter Assays

Cignal Reporter Assay Kit: A High Performance Tool for Assessing the Functions of Genes, Biologics, and Small Molecule Compounds
This paper describes the development of the Cignal Reporter Assays, and evaluates their performance. Application examples highlighting the utility of these assays for understanding gene function, as well as determining mechanisms of action of proteins, peptides, ligands, and small molecule compounds are presented.